Figure 4
From: The effects of conjugate and light dose on photo-immunotherapy induced cytotoxicity
Correlation between the concentration of Pan-IR700 and light dose for cell killing. (A) At as constant light dose of 2 J/cm2, as the concentration of Pan-IR700 increased from 0.3 to 3 μg/mL, the percentage of dead cells increased. There was a positive linear correlation on a log scale between increasing dose and cell killing at a constant light dose (R2 = 0.94). Note, that above 3 μg/mL no further increases in cell death are seen due to saturation of the membrane antigens. (B) The same rate of cell killing (measured by bioluminescence imaging) could be achieved by any of the following combinations: (1) 3 μg/mL and 0.5 J/cm2, (2) 1 μg/mL and 1.5 J/cm2, and (3) 0.3 μg/mL and 5 J/cm2) resulting in no significant difference among these three groups (p > 0.05). (C) The correlation between light dose and the concentration of Pan-IR700 derived the following equation: [concentration of Pan-IR700] = 9.65e^-1.15 [irradiation dose]. The product of the light dose (in J/cm2) and the concentration of Pan-IR700 (in μg/mL) is a constant of 1.5.