Figure 1

Immunohistochemical staining of ERα and ERβ1 in PTC lesions and NTG tissue (400×). ERα and ERβ1 expression was analyzed by immunohistochemical staining of formalin-fixed and paraffin-embedded thyroid tissue sections from patients with papillary thyroid cancer (PTC) and those with nodular thyroid goiter (NTG) using specific antibodies and the Elivision™ plus two-step system. (A) NTG tissue with negative ERα staining; (B) NTG tissue with positive nuclear ERα staining (total score 6); (C) PTC tissue with negative ERα staining; (D) PTC tissue with positive nuclear ERα staining (total score 8); (E) PTC tissue with positive nuclear and cytoplasmic ERα staining (total score 7); (F) NTG tissue with negative ERβ1 staining; (G) NTG tissue with positive nuclear ERβ1 staining (total score 8); (H) NTG tissue with positive nuclear and cytoplasmic ERβ1 staining (total score 7); (I) PTC tissue with negative ERβ1 staining; (J) PTC tissue with positive nuclear ER β1 staining (total score 6); (K) PTC tissue with positive nuclear and cytoplasmic ERβ1 staining (total score 7); (L) PTC tissue with positive cytoplasmic ERβ1 staining (total score 5).