In vivo proliferation and metastasis assay. (A1-A3) SGC-7901 cells transfected with LV-FOXO4 or LV-control were transplanted under the skin. Six weeks later, tumors were more clearly seen in mice implanted with 7901-NC cells as compared to the 7901-FOXO4 groups: (6/6 in the 7901-NC groups and 3/6 in 7901-FOXO4 groups). The tumors were dissected and measured. (B1-B3) SGC-7901 cells transfected with LV-FOXO4 or LV-control were injected into the tail veins of nude mice. Ten weeks later, mice implanted with 7901-NC cells showed lung and liver metastases, whereas few metastases were detected in mice implanted with 7901-FOXO4 cells: (for lung metastasis, 6/10 in the 7901-NC groups and 1/10 in the 7901-FoxO4 groups, For liver metastasis, 3/10 in 7901-NC groups and 0/10 in 7901-FoxO4 groups. (C) Images showing representative hematoxylin and eosin staining of lung and liver tissue samples from the different experimental groups *P < 0.05. (D) Overall survival of the nude mice in each group.