Effect of FOXO4 in regulating GC cell metastasis. (A1-A2) Up-regulation of FOXO4 expression in LV-FOXO4 cells decreased SGC-7901 cell migration and invasion in vitro, whereas the inhibition of FOXO4 expression using the oligo nucleotide inhibitor of FOXO4 enhanced BGC-823 cell migration and invasion. (B) Cell migration capacity was evaluated by performing a high-content assay in SGC-7901 cells transfected with LV-FOXO4 or LV-control. *P < 0.05 (C) Cell migration capacity was also tested by performing a wound-healing assay in SGC-7901 cells transfected with LV-FOXO4 or LV-control. *P < 0.05.