Figure 2

Effect of FOXO4 on regulating GC cell proliferation. (A1-A2) Relative expression of FOXO4 in SGC-7901 cells transfected with LV-FOXO4 or LV-control, which was confirmed by real-time PCR and western blot analysis. The values represent the means from three separate experiments, and the error bars represent the SEM (**P < 0.01). (A3) The proliferation rates of cells were measured using the MTT assay. The values represent the means from three separate experiments, and the error bars represent the SEM (*P < 0.05). (B1-B2) Relative expression of FOXO4 in BGC-823 cells transfected with FOXO4 oligo nucleotide inhibitor or oligo nucleotide control, which was confirmed by real-time PCR and western blot analysis. The values represent the means from three separate experiments, and the error bars represent the SEM (**P < 0.01). (B3) The proliferation rates of cells were measured using the MTT assay. The values represent the means from three separate experiments and the error bars represent the SEM (*P < 0.05). (C1-C2) Colony formation of SGC07901 cells transfected with LV-FOXO4 and LV-control was carried out by seeding cells onto plates for 2 weeks, and the number of colonies was then counted. The values represent the means from three separate experiments, and the error bars represent the SEM (*P < 0.05). (D1-D2) Cell cycle distribution of SGC-7901 cells transfected with LV-FOXO4 or LV-control. Cell cycle analysis was performed 24 h after transfection. The cell cycle distribution was calculated and expressed as the mean ± SD of three separate experiments. *P < 0.05. (E1-E2) Relative expression of CyclinD1 in SGC-7901 cells transfected with LV-FOXO4 or LV-control, which was confirmed western blot analysis. The values represent the means from three separate experiments, and the error bars represent the SEM(*P < 0.05).