Figure 3
From: Alcohol induces cell proliferation via hypermethylation of ADHFE1 in colorectal cancer cells
Changes in methylation and expression of ADHFE1 by ethanol treatment. The methylation and expression changes of ADHFE1 are determined in HT-29, SW480, and DLD-1 cells by treatments with ethanol using QMSP, real-time PCR and immunoblot analysis. A. The methylation status of ADHFE1 is significantly increased by ethanol treatment in 2 CRC cells. B. ADHFE1 expression is diminished by ethanol in 3 CRC cells. C. After treatment with ethanol in CRC cells, the protein expression of ADHFE1 is decreased in a concentration-dependent manner. Expression of GAPDH is used as a loading control. *p-Values of < 0.05 were considered statistically significant; PMR: Percentage of methylated reference.