Figure 8
The expression and localization of various β-tubulin isoforms in selected MCF-7 cells. (A) The expression of α-tubulin and various β-tubulin isoforms including β-1, β2, β3, and β-4 tubulin in MCF-7CC, MCF-7TXT and MCF-7DOX cells was determined by immunoblotting with specific antibodies. (B) Quantification of the data from three independent experiments as described in panel A. The intensity of the bands of various tubulin proteins was normalized against the intensity of the actin loading. The error bar is standard error. ** indicates that the difference is statistically significant with p < 0.01. (C) Immunofluorescence. The expression and the localization of various tubulin proteins including α-, β1-, β2-, β3-, and β4- tubulin in MCF-7CC, MCF-7TXT and MCF-7DOX cells was determined by indirect immunofluorescence. The red indicates the various tubulin proteins. Size bar: 10 μm.