Figure 7
The effects of docetaxel on the microtubule dynamics of MCF-7 TXT an MCF-7 CC cells. The Live imaging was performed as described in Methods. Following the transfection of the cells with GFP-tagged α-tubulin for 24 hours, the cells were incubated with docetaxel of indicated concentration for 1 hour. The images of microtubule dynamics of MCF-7CC and MCF-7TXT cells were recorded every 2 seconds by live imaging. The extension rate (A) and shortening rate (B) of microtubules were measured from the recorded images. Each datum is the average of 20 measurements from at least 8 different cells. The error bar is the standard error. * indicates that the difference is statistically significant with p < 0.05. ** indicates that the difference is statistically significant with p < 0.01. (C) Selected images from the live imaging (Additional file 3: Video S8&9) of microtubule dynamics of MCF-7CC and MCF-7TXT cells following treatment with 0.5 μM docetaxel for 1 hour. Arrow indicates the extending microtubules. Arrow head indicates the shortening microtubules. Size bar, 10 μm.