Figure 1
Platelets antagonism of Sorafenib or Regorafenib mediated cell growth inhibition. Hep3B (A, B), PLC/PRF/5 (C, D) and HepG2 (E, F) cell lines were cultured in 1% FBS medium in presence of different platelet concentrations or FBS and incubated with 1–2.5 μM Sorafenib (A, C, E) or Regorafenib (B, D, F). MTT assay was assessed after 24-72 h. (G) MTT assay performed on PLC/RFP/5 cells treated or untreated with 2.5 μM Regorafenib and cultured in different FBS concentrations (0-5%) for 48 h in presence or absence of hPL derived from 3.75 × 107 platelets. (H) AFP levels in the cell culture medium of PLC/PRF/5 cell lines containing hPL or FBS after treatment with different Sorafenib concentrations. The results are expressed as mean ± SD. *p < 0.05; **p < 0.001; ***p < 0.0001.