MiR-133b is downregulated in GC tissues and cells. (A) Relative expression of miR-133b in 140 GC patient tissues compared with adjacent non-tumor tissues. qRT-PCR results are shown as -△△CT values. (B) The boxes represent the distribution of miR-133b expression from the 25the to 75th percentiles of all patient samples. The whiskers represent distribution from the 10th to 90th percentiles, with the horizontal lines showing the median levels of miR-133b. ***, p <0.001. (C) Relative expression of miR-133b in eight GC cell lines and one immortalized normal gastric mucosal epithelial cell line (GES-1). qRT-PCR results are the mean of three independent experiments ± S.D. *, p <0.05, **, p <0.01.