Figure 4

TAMs promote the invasive activity of LoVo-P cells through KCNN4. A) Transwell chamber assays for LoVo-P cells and LoVo-C cells cocultured with TAMs for 0, 12, 24, or 36 h. B) Bars correspond to the mean ± SD, **p < 0.01. The numbers of cells passed through the Matrigel matrix. C) The ratio of LoVo cells cocultured with/without TAMs. D) TAMs were pretreated with or without BAY11-7082, and then cocultured in transwell chamber assays with LoVo-P cells. Bars correspond to the mean ± SD, **p < 0.01, compared with no BAY11-7082 treatment. E) Transwell chamber assays for LoVo-P cells cocultured with TAMs (Control) or cocultured with TAMs pretreated with p50-siRNA, p65-siRNA and Lipofectamine 2000. Bars correspond to the mean ± SD, **p < 0.01, compared with Control (non-transfected) and Lipofectamine 2000.