Figure 3

Activation of YB-1 suppresses Sox2 expression. Western blots depicting (A) altered PI3K/Akt/YB-1 signaling after 0, 10, or 30 μM treatments of LY294002 (PI3K inhibitor) for 6 hours, (B) altered YB-1 phosphorylation after 24-hour treatments of 50 μM SL0101 (RSK1/2 inhibitor) and 10 μM CHIR99021 (GSK3ß inhibitor), (C) Sox2 protein expression after 72-hour treatment of 10 μM CHIR99021, and (D) altered PI3K/Akt/YB-1 signaling after 6-hour 0, 25, or 100 ng/mL IGF-1 stimulations in MCF7 RU and RR cells. Accompanying q-RT-PCR graphs of SOX2 transcript levels are shown. DMSO only treatments were used as vehicle controls except for D where PBS only treatments were used.