Figure 4

Metformin increases lactate production, intracellular NAD(P)H and low molecular weight reduced thiols in OSCC cells. A, Increased secretion of lactate (per 10⁴ cells) indicated increased glycolysis levels in OSCC cells treated with 10 mM metformin for 24 hours in comparison to untreated cells (n = 3, mean ± SD). B, Elevated total reducing equivalents (per 10⁴cells) in OSCC cells treated with 10 mM metformin for 24 hours in comparison to untreated cells (n = 3, mean ± SD). C, Low molecular weight thiols levels (per 10⁴ cells) is higher in OSCC cells treated with 10 mM metformin for 24 hours, in comparison to untreated cells (n = 3, mean ± SD).