The role of Akt1 in the glucosamine-induced regulation of cell proliferation in H226B cells. (A) The indicated cells were incubated with 0.5 ~ 10 mM glucosamine for 3 days and the MTT assay was performed. (B) An MTT assay was carried out under low and high serum conditions. *P < 0.05 compared with H226B-Babe cells. (C) Adherent and floating cells were analyzed using western blotting.