Figure 2

qRT-PCR analysis of phenotype-specific miRNAs in NB cell lines. The cell line panel includes six N-type [SH-SY5Y (1), SMS-LHN (2), BE(2)-M17V (3), LA1-55n (4), KCN-83n (5), SK-N-BE(1)n (6)]; four I-type [CB-JMN (7), BE(2)-C (8), SK-N-LD (9), SK-N-HM (10)]; and three S-type cell lines [SH-EP1 (11), SMS-KCNs (12), and LA1-5s (13)]. Levels of S-cell specific (A) miR-21, (B) -221 and (C) -335 were normalized to loading control U6 and expressed as a fold change compared to a standard sample of LA1-5s. Levels of (D) miR-124 and (E) -375 were normalized to loading control U6 and expressed as a fold change compared to a SH-SY5Y standard. Each bar represents the mean ± SEM of 3 or more samples.