Figure 1

Expression of GD2 on the cell surface of EL-4, IMR-32, and mS tumor cell lines. Flow cytometry analysis of the cells stained with anti-GD2 antibodies conjugated with AlexaFluor488 (14G2a antibodies; 5 μg/ml; see Methods) is shown in (A). Filled histograms (red color) show staining with anti-GD2 mAbs, empty histograms – staining with an isotype control. Confocal imaging of EL-4, IMR-32, and mS cells stained with anti-GD2 conjugated with AlexaFluor488 (14G2a antibodies; 5 μg/ml; see Methods) is shown in (B). The staining with anti-GD2 mAb is shown in green color; the nuclei were counterstained with Hoechst 33342 (shown in blue). Bar scale: 50 μm.