Effect of ascites on HPMC proliferation. (A) HPMCs were cultured either with 10% FBS, 10% benign peritoneal fluids or 10% malignant ascites in the presence or absence of hydroxyurea (HU) for 12 h and phase contrast images were taken (x 200 magnification). Bars 100 μm. (B-C) Phase contrast pictures of Meso-7 and Meso-9 cells cultured either with 10% benign fluid (OV370 or OV401) or 10% malignant ascites (OVC508 or OVC509). Bars 100 μM. (D) HPMC (meso-7 cells) were seeded and cell growth for up to 96 h was determined by XTT assay. (E) Determination of LPA levels in benign fluids or malignant ascites. There was no significant difference between levels of LPA in OV401, OVC508 and OVC509 (P > 0.05). Levels of LPA in OV370 were however significantly higher. *indicate P < 0.01, T-student test.