Expression of microarray candidate genes in response to RU-486 treatment. EPH-4 cells were treated 24 hours after plating (ie. at 0 hrs) with either ethanol vehicle (UT) or 10 μM RU-486 (+RU-486) in serum-free media for a period of 48 hours, after which RNA was prepared. qRT-PCR analysis of gene expression was conducted using TaqMan mouse gene expression assays for Brca1, Hsd11b1, and Ch25h. Raw C
t values for each gene were normalized to raw C
t values for mouse Tbp internal control for triplicate samples, and are presented as the level of expression relative to the UT sample. Bars represent the mean of technical replicates, and error bars represent standard deviation (N = 3).