Expression of microarray candidate genes in response to HC treatment. EPH-4 cells were treated 24 hours after plating (ie. at 0 hrs) with either ethanol vehicle (-HC) or 1 μg/mL HC (+HC) in serum-free media for a period of 48 hours. RNA was prepared at 0, 24, and 48 hours, and qRT-PCR analysis of microarray candidate gene expression was conducted using TaqMan mouse gene expression assays for each gene: A. Brca1
B. Hsd11b1, C. Ch25h, D. Ces1, E. Oas2, F. Slc5a9, and G. Nr3c1. Raw C
t values for each gene were normalized to raw C
t values for mouse Tbp internal control for triplicate samples, and are presented as the level of expression relative to the -HC sample at 0 hrs. Bars represent the mean of technical replicates, and error bars represent standard deviation (N = 3). Statistically significant changes in gene expression relative to the -HC sample for each time point are indicated for each gene: one asterisk, p < 0.05 (significant); two asterisks, p < 0.005 (very significant); three asterisks, p < 0.0005 (very highly significant).