Figure 5

Validation of microarray candidate genes. qRT-PCR validation of microarray candidate gene expression was conducted using RNA prepared from EPH-4 EV-50 and shGR-19 stable cells and TaqMan mouse gene expression assays for each gene: A. Hsd11b1, B. Ch25h, C. Ces1, D. Oas2, E. Slc5a9, F. Brca1, and G. Nr3c1. Raw C _t values for each gene were normalized to raw C _t values for mouse Tbp internal control for triplicate samples, and are presented as the level of expression relative to the EV-50 sample. Bars represent the mean of technical replicates, and error bars represent standard deviation (N = 3). Statistically significant changes in gene expression relative to EV-50 are indicated for each gene: one asterisk, p < 0.05 (significant); two asterisks, p < 0.005 (very significant); three asterisks, p < 0.0005 (very highly significant).