Figure 2
From: Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer
Autophagy induced by PA-MSHA in breast cancer cell lines. A. MDA-MB-231HM cells treated by PA-MSHA (10 × 108/ml) for 48 h were visualized by electron microscopy (×8400). → point to autophagosome. * indicate dilated ER cavity. B. The expression of LC3 and ATG5 in MDA-MB-231HM cells treated by different dosage of PA-MSHA (0, 2.5, 5, 10 × 108/ml). Con group: cells were treated by PA-MSHA (10 × 108/ml) + PBS. 3MA group: cells were treated by PA-MSHA (10 × 108/ml) + 3-MA (2 mM). C. The relative expression of LC3-II/LC3-I and ATG5 in MDA-MB-231HM cells treated by different dosage of PA-MSHA (2.5, 5, 10 × 108/ml) to PA-MSHA (0 × 108/ml) group and Con group. The values were the means +/- standard deviations of three independent experiments. * indicates a significant difference compared with PA-MSHA (0 × 108/ml) group (*, P <0.05) . ** indicates a significant difference compared with Con group (**, P < 0.05). D. Punctate fluorescent patterns of EGFP-LC3 observed by fluorescence microscope in MDA-MB-231HM cells treated with PA-MSHA (10 × 108/ml) for 24 hours. E. The percentage of EGFP-LC3 dots positive cells to the overall MDA-MB-231HM cells. Cells were treated with PA-MSHA (10 × 108/ml) for 24 hours. The values were the means +/- standard deviations of three independent experiments. Asterisks indicate a significant difference from control cells (*, P <0.05) .