Figure 2

Comparison of STAT1 expression levels as determined by IHC, RT-PCR and immunoblotting. Total STAT1 immunostaining scores for either stroma or tumor epithelium obtained with paraffin-embedded material were correlated with mRNA expression and protein expression levels in tumor extracts. Both total STAT1 as well serine (S727) or tyrosine (Y701) phosphorylated STAT1 was determined by Western blotting. Correlation coefficients (R) and significance levels (p) were calculated by Spearman’s rank correlation and are represented by color coding.