Figure 1

Immunohistochemical (IHC) detection of NILCO and targets in tissue arrays from breast cancer and non-malignant patients. Representative pictures of immunohistochemical staining from breast cancer TNBC (A), ER+ (B), ER- (C) and non-malignant tissues (D) and, IHC negative controls (E) for the expression of NILCO and targets. Arrows show positive staining of NILCO components: Notch1 (a), Notch4 (b), DLL4 (c), JAG1 (d), leptin (e), OB-R (f), IL-1R tI (g) and targets VEGF (h) and VEGFR2 (i), respectively. No staining was found in IHC-negative controls using anti-mouse (j), anti-rabbit (k) and anti-goat (l) secondary antibodies. Magnification ×10.