Figure 5

Ceramide does not alter Fas receptor expression. A. Cell surface Fas protein level. SW620 cells were cultured in the presence of LCL85 (5 μM) and Vorinostat (0.5 μM) for approximately 24 h and analyzed for cell surface Fas protein level by flow cytometry. Gray area: IgG isotype control staining, solid line: Fas-specific mAb staining. B. Quantification of Fas protein level. Cells as shown in A were quantified for Fas protein level by mean fluorescence intensity (MFI). Column, mean; bar, SD. C. SW620 cells were cultured in the presence of C16 ceramide (2 μM) for approximately 24 h and analyzed for cell surface Fas protein level by flow cytometry. Gray area: IgG isotype control staining, solid line: Fas-specific mAb staining. D. Quantification of FAS MFI as shown in C. Column: mean; bar: SD.