Immunofluorescence Analysis of FAK expression in invasive breast tumour epithelium and endothelium of different ‘Intrinsic’ molecular tumour types. Formalin fixed paraffin embedded tissue was stained for Pecam-1 (red) to identify endothelial cells and for FAK (green). Cell nuclei have been identified by counterstaining with DAPI. Characteristic examples are shown from Luminal A (A–D), Luminal B (E–H), Her2-overexrpessing (I–L) and Triple Negative (M–P) invasive breast tumours. All images are on x40 magnification. The analysed vessels had a diameter ≥5 μm and vessels in a ≥100 μm distance away from the tumour cell margins have been excluded. Arrows indicate tumour cells and arrowheads analysed blood vessels.