Figure 3

Calcitriol induced ERα protein expression. SUM-229PE cells were treated with two calcitriol concentrations (Cal, 1X10^-8 M and 1X10^-7 M) and two MAPK inhibitors: U0126 (U, 10 μM) or Gefitinib (G, 0.8 μM) used as controls of ERα induction during 72 hr. Control incubations were done in the presence ethanol (C) or DMSO (D). Results were analyzed by western blots. MCF-7 (M) nuclear extracts were used as positive control for ERα and GAPDH was utilized as the loading control for normalization. Results are representative from two independent experiments.