Significant down-regulation of SDF-1 (CXCL12) expression in the metastatic niches. A and B: Levels of SDF-1 by ELISA in the lung (A) and bone (B) lysate of SKG with no tumor, 4 T1 tumor bearing SKG mice treated with control or anti-IL-17A antibody (*P < 0.05) (N = 3 mice). The limit of detection for mouse SDF-1 is 9.38 pg/ml to 600 pg/ml. C and D: Representative images of SDF-1 expression by IHC in lung (C) and bone (D) (N = 6 mice), (i) Secondary antibody only; (ii) Balb/C with no tumor or AA (iii) SKG with no tumor; (iv) 4 T1 tumor bearing SKG mice treated with control IgG antibody or (v) with anti-IL-17A antibody. Brown staining represents SDF-1 localization (Bone at 400X magnification and Lung at 600X magnification). E and F: Levels of SDF-1 by ELISA in the lung (E) and bone (F) lysate of PyV MT mice with no AA and PyV MT + AA with control or anti-IL-17A antibody treatment (*P < 0.05) (N = 3 mice).