Figure 7
Effect of siRNA knockdown of TKT or AK2 on the glutamine uptake by UM1 and UM2 cells. A significantly higher amount of glutamine was consumed by siTKT-transfected UM1 (n = 4, p = 0.01) and UM2 (n = 4, p = 0.02) cells when compared to the siCTRL-transfected cells. Similarly, siAK2-transfected UM1 (n = 4, p = 0.02) and UM2 (n = 4, p = 0.03) cells consumed a significantly higher amount of glutamine than the siCTRL-transfected cells.