Figure 5

Oridonin regulates the expression of apoptosis related proteins and caspases activities in gallbladder cancer cells. SGC996 and NOZ cells were treated with oridonin (7.5, 15, and 30 μmol/L) for 48 h. (A) The expression levels of NF-κB, Bax, Bcl-2, cleaved caspase-9, caspase-3, cleaved PARP-1, cytochrome c were detected by western blot analysis, and β-actin was used as a loading control. (B) The data of the caspases activities were expressed as mean ± SD. The results shown were representative data from 3 independent experiments. *P < 0.05, **P < 0.01 vs. the control group.