Figure 3

Oridonin induces cell cycle arrest at S-phase and regulates the expression of cell cycle-related proteins in gallbladder cancer cells. SGC996 and NOZ cells were treated with oridonin (7.5, 15, and 30 μmol/L) for 48 h. (A) The cell cycle phases of the treated cells were evaluated by flow cytometry. (B) Data were expressed as mean ± SD (n = 3). (C) The expression levels of cyclin A, cyclin B1, and cyclin D1 were measured by western blot analysis, and β-actin was used as a loading control. Results were representative of 3 independent experiments. *P < 0.05, **P < 0.01 vs. the control group.