Figure 2

MV-hE:A and MV-mE:A infection results in the secretion of active endostatin:angiostatin. (A) Human endostatin production in infected D283med and D425med cells as quantified by ELISA. Endostatin concentration is expressed in ng/ml per 10⁴ cells. (B) Western blot analysis of media taken from either MV-hE:A or MV-mE:A infected D283med and D425med probed with an anti-angiostatin antibody. (C-D) Conditioned media from MV-hE:A and MV-mE:A infected Vero cells (10 μg/ml total protein) inhibits VEGF-mediated tube formation in HUVEC cells (*p ≤ 0.05, ** p ≤ 0.001). (E) To examine the effect of MV-E:A conditioned media on migration, scratch assays were performed in HUVECs by allowing the cells to move to the scraped region for 18 hours using VEGF (10 ng/ml) as a positive control in the presence and absence of MV conditioned media (original magnification × 40). (F) A crystal violet assay was also performed as described in Materials and Methods section to quantify the effect of MV conditioned media on migration (*p ≤ 0.05).