Figure 3
The negative correlation between MAT2A and HO-1 expression. mRNA levels of HO-1 and COX-2 were analyzed with real-time qRT-PCR. The correlation analysis was performed between MAT2A and HO-1 as well as COX-2 in RCC patients. A and B Relative mRNA expression levels of HO-1 (A) and COX-2 (B) were higher in RCC cancer tissues (C) than in paired normal tissues (N) (n = 24; P < 0.05). C Relative mRNA expression level of HO-1 and COX-2 were higher in 4 RCC cell lines than in HEK293. D The western blotting analysis of MAT2A and HO-1 in cell lines.The correlation of protein content between MAT2A and HO-1 is negative. E The statistical analysis reveals a negative correlation between MAT2A and HO-1 expression in RCC patients (P < 0.01). F The statistical analysis reveals no significant correlation between MAT2A and COX-2 expression in RCC patients.