Figure 5

Effect of PAX8 knock down on A549 NSCLC cell motility and migration. A. Wound healing assay was performed in A549 cells transfected with control (scramble) and PAX8 specific siRNA for 72 h and then replated in 24 well plate containing cell culture inserts. The fine scratch created by the inserts was monitored and photographed for 12 hr. Representative pictures of the degree of wound closure in A549 cells when subjected to a scratch in control and PAX8 knock down cells at 12 hr. B. The open wound at each time point was quantified and normalized to 0 h. The experiments were done in triplicate and the average data is shown with standard error bars. C. A549 cells were transfected with either control or PAX8 specific siRNA as described in Methods. After 96 h the cells were replated in Boyden chambers for migration assay. Migrated cells were fixed and stained with Crystal violet. Representative images of control and PAX8 knockdown cells were captured using a microscope. D. The migrated cells were counted using Image J. The experiments were done in triplicate and the averages with standard error bars are shown.