Figure 4
Effect of PAX8 knockdown on A549 NSCLC cell apoptosis. A549 cells were transfected with control (scramble) and PAX8 specific siRNA at 100 nM concentration for 72 h. Apoptosis was quantified using flow cytometry after staining with Annexin V/PI and effect of loss of PAX8 expression on apoptosis is shown in the representative charts. Both early (right lower quadrant) and late (right upper quadrant) stage apoptosis increased in PAX8 knockdown cells as shown by flow cytometry analysis.