Vasculature analysis of patient derived tumor xenografts using species-specific PCR assays: evidence of tumor endothelial cells and atypical VEGFA-VEGFR1/2 signalings

Bieche, Ivan; Vacher, Sophie; Vallerand, David; Richon, Sophie; Hatem, Rana; De Plater, Ludmilla; Dahmani, Ahmed; Némati, Fariba; Angevin, Eric; Marangoni, Elisabetta; Roman-Roman, Sergio; Decaudin, Didier; Dangles-Marie, Virginie

doi:10.1186/1471-2407-14-178

Table 1 Normalized gene expression for each of the 150 PDX samples, classified by tumor type (noted in bold)

From: Vasculature analysis of patient derived tumor xenografts using species-specific PCR assays: evidence of tumor endothelial cells and atypical VEGFA-VEGFR1/2 signalings

Sample nature	Derived from primary tumor or metastatis	% of mouse cells	PECAM1		ENG		VEGFR1		VEGFR2		VEGFA		% of *mVegfa*vs human + mouse VEGFA transcripts
			Hs	Mm	Hs	Mm	Hs	Mm	Hs	Mm	Hs	Mm
Pure human control		0%	1265	0	796	0	2610	0	157	0	287	0
Pure mouse control		100%	0	1176	0	736	0	303	0	879	0	790
Colorectal carcinoma PDX
CRC#1	Primary	11%	0	894	2	492	23	453	0	405	4010	212	5%
CRC#2	Primary	5%	0	917	3	398	9	383	0	309	4912	51	1%
CRC#3	Metastasis	21%	1	2380	34	893	14	843	0	803	4642	628	12%
CRC#4	Primary	17%	0	836	<1	285	0	368	0	299	2876	302	10%
CRC#5	Metastasis	8%	0	813	0	492	3	337	0	374	3552	109	3%
CRC#6	Primary	9%	46	458	217	326	77	196	<1	176	1866	84	4%
CRC#7	Metastasis	8%	17	553	27	272	65	292	0	210	5230	251	5%
CRC#8	Primary	14%	0	1193	469	614	3	349	0	689	2999	92	3%
CRC#9	Primary	8%	0	967	8	550	3	475	0	379	7973	204	2%
CRC#10	Primary	10%	0	733	<1	409	176	246	0	284	3463	124	3%
CRC#11	Metastasis	9%	1	1083	<1	481	300	567	0	410	5461	135	2%
CRC#12	Metastasis	4%	48	479	0	182	26	274	0	230	4937	106	2%
CRC#13	Metastasis	4%	3	356	5	135	289	163	0	168	3606	145	4%
CRC#14	Primary	2%	<1	260	7	139	305	119	0	143	5085	76	1%
CRC#15	Primary	17%	<1	1287	<1	715	51	530	0	419	6541	311	5%
CRC#16	Metastasis	5%	<1	477	44	237	89	197	0	219	3406	196	5%
CRC#17	Primary	17%	21	1067	49	539	42	382	0	323	3674	555	13%
CRC#18	Primary	14%	4	1078	81	550	33	370	<1	356	2016	262	12%
CRC#19	Primary	4%	3	288	<1	162	<1	120	0	135	4258	111	3%
CRC#20	Metastasis	22%	4	1580	19	754	10	584	<1	684	5604	391	7%
CRC#21	Metastasis	17%	10	1336	373	749	10	656	0	639	4894	432	8%
CRC#22	Primary	18%	0	2315	322	1081	32	908	0	1262	4671	1244	21%
CRC#23	Metastasis	8%	0	446	407	406	42	202	0	173	2360	155	6%
CRC#24	Primary	12%	0	981	5	581	13	508	0	331	4773	233	5%
CRC#25	Primary	5%	0	622	36	329	0	246	0	285	2643	68	3%
CRC#26	Primary	11%	0	1245	569	480	112	375	0	296	3607	237	6%
CRC#27	Primary	14%	4	1789	3	895	83	682	0	581	3101	891	22%
CRC#28	Carcinosis	5%	3	526	1	326	1	215	0	268	2545	29	1%
CRC#29	Primary	11%	5	1000	2	541	0	364	0	344	3172	391	11%
CRC#30	Primary	7%	0	753	11	332	22	282	0	258	2247	231	9%
CRC#31	Metastasis	10%	<1	629	1	294	29	241	0	216	2896	210	7%
CRC#32	Primary	16%	0	1073	304	556	28	357	0	469	1731	166	9%
CRC#33	Primary	7%	4	563	<1	277	7	202	0	218	1253	129	9%
CRC#34	Primary	13%	2	749	379	530	15	306	0	390	4293	157	4%
CRC#35	Primary	9%	0	958	3	484	9	329	0	318	2206	212	9%
CRC#36	Primary	21%	1	991	0	504	32	388	<1	436	3296	140	4%
CRC#37	Primary	19%	6	1978	16	840	10	391	0	668	2692	182	6%
CRC#38	Primary	8%	2	1114	8	446	2	320	0	367	1889	218	10%
CRC#39	Metastasis	12%	0	1156	478	523	40	366	0	418	4034	214	5%
CRC#40	Primary	10%	<1	547	94	356	49	199	0	242	1848	142	7%
CRC#41	Carcinosis	16%	0	1552	3	762	7	325	0	457	918	228	20%
CRC#42	Primary	31%	0	1786	<1	922	94	447	0	599	2710	493	15%
CRC#43	Primary	10%	0	1024	75	459	249	358	2	431	4126	272	6%
CRC#44	Carcinosis	15%	<1	938	159	565	1	285	0	364	2523	269	10%
CRC#45	Primary	12%	1654	807	512	388	9	215	1	332	969	124	11%
CRC#46	Primary	3%	<1	412	3	158	2	139	0	168	1865	61	3%
CRC#47	Metastasis	6%	0	521	2	252	<1	173	0	195	1662	68	4%
CRC#48	Carcinosis	10%	0	843	<1	417	0	252	0	252	1705	227	12%
CRC#49	Metastasis	6%	1	379	426	274	11	248	0	267	4587	149	3%
CRC#50	Metastasis	18%	31	1697	0	690	23	485	0	421	5271	299	5%
CRC#51	Primary	23%	0	1294	2	662	67	476	0	375	6660	583	8%
CRC#52	Primary	38%	14	3265	398	1126	640	736	0	836	7517	953	11%
CRC#53	Metastasis	19%	0	1657	0	566	15	430	0	430	4014	209	5%
*Median*		*10.6%*	*0.7*	*958*	7	*484*	22	*349*	0	*344*	*3463*	*210*	6%
Ovarian carcinoma PDX
OVC#1	Metastasis	28%	42	2575	0	1498	89	1191	159	867	10390	459	4%
OVC#2	Metastasis	5%	4	565	99	350	2	439	34	259	6391	88	1%
OVC#3	Metastasis	21%	1	1427	304	809	69	406	<1	583	3133	710	18%
OVC#4	Primary	6%	26	709	9	474	0	259	4	272	1528	144	9%
OVC#5	Primary	7%	16	974	81	807	0	802	45	525	14226	95	1%
OVC#6	Primary	12%	3	2052	97	593	19	734	101	528	2628	427	14%
OVC#7	Primary	8%	0	762	4	470	0	270	32	278	6156	266	4%
OVC#8	Primary	3%	2	219	30	119	6	88.8	3	59.2	652	37	5%
OVC#9	Primary	8%	5	1795	2	674	3	518	5	372	2981	184	6%
OVC#10	Primary	4%	1	444	16	288	0	204	22	141	2812	52	2%
OVC#11	Primary	20%	24	1586	54	1036	0	482	2	648	2781	493	15%
OVC#12	Primary	13%	3	877	177	487	0	259	12	285	1720	127	7%
OVC#13	Primary	3%	17	550	207	263	2	196	<1	224	1134	16	1%
OVC#14	Primary	5%	0	332	<1	255	21	238	<1	164	19239	62	0%
*Median*		7%	*3.7*	*819*	42	*480*	2	*338*	9	*281*	*2896*	*136*	5%
Glioblastoma PDX
GBM#1	Primary	8%	22	712	2051	457	378	559	8	186	18822	241	1%
GBM#2	Primary	13%	1	1351	1143	819	0	799	378	328	17084	296	2%
GBM#3	Primary	13%	1	2372	422	1184	0	1325	0	1237	8452	131	2%
GBM#4	Primary	5%	55	870	321	328	0	503	0	372	5923	78	1%
GBM#5	Primary	15%	0	2600	268	1389	28	1361	294	1413	15443	100	1%
*Median*		*13%*	*1.4*	*1351*	*422*	*819*	0	*799*	8	*372*	*15443*	*131*	1%
Breast cancer carcinoma PDX
BC#1	Primary	2%	<1	222	204	113	3	89.4	2	80.7	637	114	15%
BC#2	Primary	8%	0	666	177	335	19	289	0	162	2997	310	9%
BC#3	Metastasis	10%	0	679	286	447	5	539	36	259	4961	334	6%
BC#4	Primary	15%	46	803	91	498	5	366	0	222	3547	447	11%
BC#5	Primary	1%	<1	116	0	61.9	33	89.1	4	31.9	15066	77	1%
BC#6	Metastasis	15%	2	1351	289	634	29	719	150	439	17360	357	2%
BC#7	Primary	22%	8	1908	442	887	0	1370	0	739	27659	365	1%
BC#8	Primary	6%	1	810	149	412	18	327	9	280	8360	160	2%
BC#9	Metastasis	10%	0	713	6	322	13	420	0	279	1020	294	22%
BC#10	Primary	6%	3	370	460	233	0	325	17	134	7447	154	2%
BC#11	Primary	6%	6	993	347	403	29	461	68	325	14282	256	2%
BC#12	Primary	8%	6	1005	466	543	28	664	3	391	25794	363	1%
BC#13	Primary	7%	0	575	92	256	8	266	15	189	6174	132	2%
BC#14	Primary	8%	654	745	45	413	0	279	2	253	3294	71	2%
BC#15	Metastasis	11%	4	912	50	461	1	311	0	286	3458	186	5%
BC#16	Primary	2%	0	199	188	94.6	4	73.6	2	69.7	610	100	14%
BC#17	Primary	4%	13	413	346	134	66	173	32	101	2131	197	8%
BC#18	Primary	10%	<1	1545	168	743	3	788	11	382	6550	167	2%
BC#19	Metastasis	16%	<1	2304	167	1188	5	1049	10	771	6004	280	4%
BC#20	Primary	17%	0	1967	340	959	1	709	20	634	11533	357	3%
BC#21	Primary	9%	0	730	334	332	2	476	91	202	8166	520	6%
BC#22	Primary	6%	0	598	451	222	10	334	90	124	5088	264	5%
BC#23	Primary	4%	0	377	331	179	2	195	19	83.3	1742	51	3%
BC#24	Primary	22%	1	1128	858	982	79	999	<1	495	21363	668	3%
BC#25	Primary	10%	0	1165	666	573	94	627	12	474	14542	244	2%
BC#26	Primary	10%	0	1446	429	572	0	685	230	510	5771	257	4%
BC#27	Primary	14%	2	880	4	452	94	415	<1	222	1505	299	17%
BC#28	Primary	5%	<1	182	91	113	7	119	6	80.3	1221	50	4%
BC#29	Primary	9%	<1	656	530	469	32	532	9	473	50360	255	1%
BC#30	Primary	7%	3	823	94	341	247	403	34	244	9097	373	4%
BC#31	Primary	4%	0	345	166	216	0	161	3	145	1085	79	7%
BC#32	Primary	7%	<1	629	13	276	4	237	19	194	1544	198	11%
BC#33	Primary	9%	<1	725	397	428	232	549	6	231	5414	144	3%
BC#34	Primary	14%	5	1061	245	557	0	457	0	308	3866	176	4%
BC#35	Primary	5%	2	484	103	358	3	506	50	185	23896	360	1%
BC#36	Primary	13%	0	1085	221	544	0	333	13	347	1153	231	17%
BC#37	Primary	4%	0	376	193	149	<1	144	13	120	1081	289	21%
BC#38	Primary	6%	2	776	90	415	8	326	12	281	4683	178	4%
BC#39	Primary	14%	0	961	5	731	83	691	4	606	4829	152	3%
*Median*		*7.9%*	*0.7*	*745*	*193*	*413*	5	*403*	10	*253*	*5088*	*244*	4%
Cutaneous melanoma PDX
CM#1	Metastasis	11%	0	1725	2985	825	0	953	83	1026	10386	1401	12%
CM#2	Metastasis	7%	27	544	1391	426	768	360	0	184	10696	315	3%
CM#3	Metastasis	4%	1	282	257	180	0	122	1	155	599	102	14%
CM#4	Primary	20%	0	3306	784	1178	427	883	38	836	9188	718	7%
CM#5	Metastasis	8%	9	936	872	363	15	587	0	289	5590	201	3%
CM#6	Metastasis	3%	<1	342	648	196	6	188	5	236	960	23	2%
CM#7	Metastasis	1%	2	176	382	83.6	2	135	<1	84.3	5962	37	1%
CM#8	Primary	10%	9	4760	876	705	0	2230	5	841	16732	239	1%
CM#9	Metastasis	1%	0	118	284	61.6	20	125	14	73.6	3704	24	1%
CM#10	Primary	10%	0	876	756	300	0	248	279	285	387	126	25%
CM#11	Metastasis	8%	2	641	1102	427	0	355	1	309	8837	266	3%
CM#12	Primary	5%	0	530	112	186	0	440	0	423	683	26	4%
CM#13	Metastasis	2%	<1	243	145	101	<1	116	1	82.2	466	31	6%
*Median*		*7.1%*	*0.9*	*544*	*756*	*300*	*0.8*	*355*	1	*285*	*5590*	*126*	3%
Renal cell carcinoma PDX
RCC#1	Primary	17%	0	1179	569	1002	0	680	2	473	19769	1513	7%
RCC#2	Primary	12%	0	3362	16	1929	0	1934	0	3043	27096	54	0%
RCC#3	Primary	27%	0	5431	411	2376	0	1866	0	1974	25792	211	1%
RCC#4	Metastasis	11%	120	2117	256	1430	4	1512	0	1337	13968	89	1%
RCC#5	Primary	16%	5	2906	33	1942	3	1624	0	2102	25817	87	0%
RCC#6	Primary	1%	1	341	2	125	2	157	47	119	609	13	2%
RCC#7	Primary	21%	0	768	549	1908	0	1292	0	1324	27232	157	1%
RCC#8	Metastasis	17%	1	842	410	778	0	466	0	286	1756	930	35%
RCC#9	Metastasis	13%	17	2024	230	1258	3	827	1	904	37839	55	0%
RCC#10	Primary	11%	0	2010	856	1359	0	1070	2	672	37217	83	0%
RCC#11	Primary	5%	2	597	907	350	0	487	0	253	5091	136	3%
RCC#12	Metastasis	14%	0	2546	257	1132	0	871	0	1040	16952	61	0%
RCC#13	Primary	21%	0	4963	38	3466	0	3281	0	3966	30645	155	1%
RCC#14	Primary	6%	330	1338	364	602	0	661	2	343	26952	52	0%
RCC#15	Primary	6%	77	565	1036	293	0	368	0	291	2210	59	3%
*Median*		*12.9%*	*1.2*	*2010*	*364*	*1258*	0	*871*	0	*904*	*25792*	87	1%
Lung carcinoma PDX
*Small cell lung carcinoma*
SCLC#1	Primary	8%	0	1030	3	387	0	250	0	196	419	43	9%
SCLC#2	Primary	3%	2	632	0	238	5	189	0	185	1300	52	4%
SCLC#3	Primary	4%	4	591	0	259	1	232	2	232	1117	49	4%
SCLC#4	Primary	3%	7	395	0	222	0	166	0	162	1498	46	3%
SCLC#5	Metastasis	2%	0	309	1	153	0	160	2	122	893	56	6%
SCLC#6	Primary	7%	2	670	9	221	471	208	72	192	954	86	8%
*Median*		*3.3%*	*1.7*	*612*	0	*230*	1	*198*	1	*189*	*1035*	51	5%
*Non small cell lung carcinoma*
NSCLC#1	Primary	28%	3	1969	61	941	2	1145	14	637	18440	794	4%
NSCLC#2	Primary	8%	0	1270	0	611	0	511	335	639	5911	98	2%
NSCLC#3	Primary	22%	95	1590	31	1438	124	961	930	669	18346	429	2%
NSCLC#4	Primary	5%	2	686	5	339	4	212	59	221	875	85	9%
NSCLC#5	Primary	20%	3	1363	667	1387	184	896	3106	652	10612	688	6%
*Median*		*20%*	*2.7*	*1363*	31	*941*	4	*896*	*335*	*639*	*10612*	*429*	4%

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ISSN: 1471-2407

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