Colony formation and scratch-wound healing assay. Colony formation assays of ShCCND1 revealed a decrease in colony number compared to that of ShScramble (A). The wound area of ShCCND1 was significantly increased compared to the wound area of ShScramble measured by the scratch-wound healing assay (B). The data presented are the mean ± SEM. ***P < 0.001 versus the ShScramble-transduced cells.