Figure 4

Platelet depletion reduced hypoxia, HIF-1α expression, and Met activation. (A) Hypoxia was detected by immunohistochemistry staining of pimonidazole adducts in B16/F10 tumor sections from control and PLT-depleted mice. Nuclei were stained with hematoxylin. Hypoxia was quantified as the percent hypoxic area per visual field. (B) Western blot analysis of HIF-1α expression in tumors from control and PLT-depleted mice. Quantification of western blot analysis for HIF-1α (normalized to β-actin). (C) Western blot analysis of p-Met, total Met, and β-actin expression in tumors from control and PLT-depleted mice. Quantification of western blot analysis for total Met (normalized to β-actin) and p-Met (normalized to β-actin).