Figure 1

Platelet depletion showed no change in tumor growth but reduced lung metastasis. (A) Orthotopic implantation of B16/F10 tumor cells into C57B6/L mice followed by injections every 3 days of GPI or control antibody after the tumors reached ~500 mm³. (B) Total tumor volumes at the experimental endpoint (24 days). (C) Representative images of H&E-stained lung sections. Scale bar, 5 μm. Arrows point to metastatic areas. Number of metastatic lung nodules in the lungs of these mice. Error bars display mean ± SEM; asterisks denote significance (*p < 0.05). (D) Western blot analysis of p-Met, total Met, and β-actin expression in tumors from control and PLT-depleted mice. Quantification of western blot analysis for total Met (normalized to β-actin) and p-Met (normalized to β-actin), Error bars display mean ± SEM; asterisks denote significance (*p < 0.05) (n = 6 for each group).