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Table 2 TNFα + WT-Ras up-regulate CXCL8 expression via the activation of AP-1

From: The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells

 

Control cells

WT-Ras cells

 

WT CXCL8 promoter

AP-1 mutated CXCL8 promoter

WT CXCL8 promoter

AP-1 mutated CXCL8 promoter

 

–

TNFα

–

TNFα

–

TNFα

–

TNFα

Exp #1

1.00

1.56

0.03

0.12

0.72

2.42

0.05

0.14

Exp #2

1.00

5.40

0.10

0.42

1.79

6.11

0.12

0.48

Exp #3

1.00

3.33

0.11

0.30

1.05

4.94

0.07

0.16

  1. MCF-7 cells were transfected with WT-Ras vector or with control vector, and were not-stimulated or stimulated by TNFα (50 ng/ml). Stimulation of the transcriptional activity of AP-1 was determined in cells transfected to express the luciferase gene under the control of WT AP-1, or mutated AP-1 binding sites in the CXCL8 promoter, using dual luciferase assay. Control vector-transfected non-stimulated cells were given the value of 1. The table presents the results obtained in 3 independent experiments. Please see “Methods” for additional details on the experimental procedures performed in this part of the study.
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BMC Cancer

ISSN: 1471-2407

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