Figure 6

Increase in the expression and translocation of AIF on treatment with MK-2206 mediates cell death: A) western blot analysis showing an increase in the expression of AIF on treatment with MK-2206. Immunofluorescence was performed to study the translocation of AIF from mitochondria to the nucleus during cell death. B) Confocal images showing a reduced co-localization of mitotracker (red) and AIF (green) in treated as compared to control cells. C) Cellular fractionation to separate nucleus from cytosol was performed followed by western blot analysis for AIF. HDAC1 and GAPDH were used as compartmentalization control for nucleus and cytosol respectively. D) DNA fragmentation after treatment with AIF inhibitor results in reduction in cell death in presence and absence of MK-2206 thus confirming that MK-2206 causes AIF mediated cell death.