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Figure 3 | BMC Cancer

Figure 3

From: Nuclear location of tumor suppressor protein maspin inhibits proliferation of breast cancer cells without affecting proliferation of normal epithelial cells

Figure 3

A tissue culture model system for studies of location-dependent maspin’s effect on breast cancer cells. a - pReceiver M03 plasmid map in which the sequence of maspin (NM_002639) was cloned; b - sequences of primers for mutagenesis to the nuclear localization signal (NLS) insertion; c - transient transfection of MCF-7 cells with three plasmids: pEGFP, Msp-EGFP and Msp-NLS-EGFP, maspin staining pattern confirms proper localization in transfected cells; d - stable cell lines: MCF-7 EGFP clone 1.2 expressing EGFP, MCF-7 maspin-EGFP clone 3.6 expressing maspin with fusion protein EGFP localized in cytoplasm; generation of cell line with nucleus-localized maspin failed because of death of all transfected cells.

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