Figure 2

Phospho-aspirin-2 inhibits the growth of TNBC xenografts. A: Chemotherapeutic effect of PA-2 on subcutaneous MDA-MB-231 xenografts in nude mice. Two representative tumors from each group are shown. *, p < 0.001, compared to vehicle; n = 10-16 tumors/group. B: Chemotherapeutic effect of PA-2 on subcutaneous BT-20 xenografts in nude mice. *, p < 0.01, compared to vehicle; n = 10-16 tumors/group. C: Chemopreventive effect of PA-2. Nude mice bearing orthotopic xenografts of MDA-MB-231 cells were treated with PA-2 or aspirin for 9 wks, starting 1 wk before cell implantation. The tumor volumes of the orthotopic MDA-MB-231 xenografts at sacrifice were determined by luciferase in vivo imaging as described in the methods section. Representative tumors from each group are shown. *, p < 0.05, compared to vehicle. D: Cytokinetic effect of PA-2 in MDA-MB-231 xenografts (treatment protocol). Left: Representative images (top) and the quantification (bottom) of Ki-67 expression in tumor sections, * p < 0.01. Right: Representative images (top) and the quantification (bottom) of TUNEL positive cells in tumor sections, * p < 0.002. All values are mean ± SEM.