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Figure 4 | BMC Cancer

Figure 4

From: Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAFmutations

Figure 4

Effects of different extracts on the same sample. DNA from a melanoma sample (case 7) was extracted and showed wildtype results using Sanger, HRM and NGS (3% mutant allele frequency). In contrast, immunohistochemistry was positive. The second extract exhibited a p.V600E mutation with all evaluated methods. Sanger: Sanger sequencing, HRM: high resolution melting analysis, NGS: next generation sequencing, IHC: immunohistochemistry.

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BMC Cancer

ISSN: 1471-2407

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