Effects of different extracts on the same sample. DNA from a melanoma sample (case 7) was extracted and showed wildtype results using Sanger, HRM and NGS (3% mutant allele frequency). In contrast, immunohistochemistry was positive. The second extract exhibited a p.V600E mutation with all evaluated methods. Sanger: Sanger sequencing, HRM: high resolution melting analysis, NGS: next generation sequencing, IHC: immunohistochemistry.