Knockdown of WNT5B led to decreased functional change in MDA-MB-231 cells. (A) Morphology alteration 3 days after shWNT5B was expressed. (B) Cell growth curve with WNT5B knockdown compared to control cells. Each point represents cell number as the mean ± SD (n = 3). Cells were observed for 5 days. ***P < 0.001 (C) ShWNT5B inhibited wound healing in MDA-MB-231 cells. Wound healing status at 0, 16, 24 and 40 h after wounding was photographed in MDA-MB-231 cells transduced with shWNT5B or shCtl. ShWNT5B attenuated mammary sphere formation in MDA-MB-231 cells. A representative mammary sphere image was taken (Left panel), and the sphere size greater than 50 um was enumerated. Scale bar: 20 um; the graph represents an average of 3 independent experiments. P = 0.0094, **P < 0.01 (Right panel).