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Table 2 Effect of PIK3CA mutations, ARID1A mutations, and BAF250a Expression on Akt phosphorylation

From: A functional proteogenomic analysis of endometrioid and clear cell carcinomas using reverse phase protein array and mutation analysis: protein expression is histotype-specific and loss of ARID1A/BAF250a is associated with AKT phosphorylation

Test variable N Univariate testing Multivariate testing
   Mean (SD) Iogeodds ratio/p-value
   pAKT-Thr308 pAKT-Ser473 pAKT-Thr308 pAKT-Ser473
ARID1A mutation      
  Yes 22 0.31 (0.64) 0.24 (0.78) 0.41 2.4
  No 33 0.06 (0.72) 0.0 (0.86) p = 0.36 p = 0.17
BAF250a expression      
  Yes 43 0.03 (0.67) 0.0 (0.82) 0.03 0.21
  No 12 0.63 (0.60)* 0.41 (0.83) p = 0.002* p = 0.04*
PIK3CA mutation      
  Helical 11 0.11 (0.81) - 0.25 (0.88) 2.2 1.3
  Kinase 8 0.50 (0.28) 0.27 (0.39) p = 0.02* p = 0.39
  Other 6 0.39 (0.82) 0.50 (0.98)   
  None 30 0.05 (0.69) 0.09 (0.86)   
PTEN level      
  PTEN low § 11 0.78 (0.97)* 0.57 (1.3) 0.1 0.24
  PTEN Normal 44 0.01 (0.51) - 0.03 (0.65) p = 0.01* p = 0.07
  1. Note: univariate mean and standard deviation values represent Iog2 relative protein expression measured by RPPA.
  2. *significant p-value (< 0.05).
  3. §lowest 20% of samples by RPPA.