A functional proteogenomic analysis of endometrioid and clear cell carcinomas using reverse phase protein array and mutation analysis: protein expression is histotype-specific and loss of ARID1A/BAF250a is associated with AKT phosphorylation

Table 2 Effect of PIK3CA mutations, ARID1A mutations, and BAF250a Expression on Akt phosphorylation

Test variable	N	Univariate testing		Multivariate testing
		Mean (SD)		Iog_eodds ratio/p-value
		pAKT-Thr³⁰⁸	pAKT-Ser⁴⁷³	pAKT-Thr³⁰⁸	pAKT-Ser⁴⁷³
*ARID1A* mutation
Yes	22	0.31 (0.64)	0.24 (0.78)	0.41	2.4
No	33	0.06 (0.72)	0.0 (0.86)	p = 0.36	p = 0.17
BAF250a expression
Yes	43	0.03 (0.67)	0.0 (0.82)	0.03	0.21
No	12	0.63 (0.60)*	0.41 (0.83)	p = 0.002*	p = 0.04*
*PIK3CA* mutation
Helical	11	0.11 (0.81)	- 0.25 (0.88)	2.2	1.3
Kinase	8	0.50 (0.28)	0.27 (0.39)	p = 0.02*	p = 0.39
Other	6	0.39 (0.82)	0.50 (0.98)
None	30	0.05 (0.69)	0.09 (0.86)
PTEN level
PTEN low ^§	11	0.78 (0.97)*	0.57 (1.3)	0.1	0.24
PTEN Normal	44	0.01 (0.51)	- 0.03 (0.65)	p = 0.01*	p = 0.07

Note: univariate mean and standard deviation values represent Iog2 relative protein expression measured by RPPA.
*significant p-value (< 0.05).
§lowest 20% of samples by RPPA.

ISSN: 1471-2407