Transwell assays of Huh7 and HCCLM3 cells transfected with control and ATAD2 siRNA. (a). ATAD2 depletion had a measurable inhibitory effect on cell invasion in both cell lines. (b). The numbers of invading cells were counted, and a significant difference was observed (*P < 0.05). (c). A significant difference was observed in both cell lines and (d) the numbers of migrating cells were counted (*P < 0.05).