Figure 3

A) Expression of miR-18b and TNRC6B according to the degree of histological differentiation in HCC. Well differentiated HCC showed low expression of miR-18b; poorly differentiated HCC showed strong expression of miR-18b. TNRC6B down-regulation in HCC is inversely related to miR-18b expression. HE stain (a, d, g), in situ hybridization of miR-18b (b, e, h) and immunohistochemistry of TNRC6B (c, f, i) are shown, respectively. Blue indicates the expression of miR-18b (arrows) and brown indicates the expression of TNRC6B (arrowheads). Bars indicate 100 μm. B) Positive cells for miR-18b in situ hybridization and TNRC6B immunostain was quantified by each miR-18b in situ hybridization and TNRC6B immunostain score system, respectively.