Transposon Mutagenesis library. A) All PB insertion sites in a PB-tagged HeLa library identified by Illumina sequencing were plotted to 23 chromosomes. X-axis indicates nucleotide positions with centromeres drawn as circles and chromosome arms as straight lines. Y-axis indicates raw read number for each site. B) Insertion sites of five clones expanded from single cells, with x-axes indicating genome positions, and y-axis indicating frequency of insertions normalized to the highest signal C) Transposon insertions alter host gene expression. Shown are four genes with PB insertions in various positions and orientations. Gene expression was compared among clones with (+ins), without (−ins) the insertion, and the prescreened library (Lib). Error bars show standard deviation (n=3). Significances were indicated by p-values.