increases TGFβ-induced EMT in LIM1863 human CRC cells. TGFβ induces EMT in LIM1863 human CRC cells in a concentration-dependent manner. Low concentration TGFβ (0.2 ng/ml) promotes LIM1863 human CRC cell colony adherence, but not spreading, unlike 2 ng/ml TGFβ, which induces an expansion in adherent colony size. PGE2 drives EMT in a concentration-dependent manner in LIM1863 cells exposed to low concentration TGFβ (0.2 ng/ml). Columns and bars demonstrate the mean and the standard error of the mean (n = 3).