Figure 1

A: Table reporting the IC50 in μmol/L for paclitaxel and cisplatin in the used panel of ovarian cancer cell lines. The cell lines are numbered progressively and the same numbers are used to indicate the lane in which each cell line was analyzed. B and C: Analysis of miR-200c and TUBB3 mRNA expression, respectively, in the cell lines numbered as in panel A. Bars and error bars refer to mean and SD of two experiments performed in triplicate. In bar charts expression was normalized on the level measured in A2780 cells (=1). D and E: Representative Western Blot for the expression of TUBB3 and HuR, respectively,in the cell lines numbered as in panel A. The Coomassie is used as loading control. The asterisk indicated the mitochondrial form of TUBB3. F: Western blot analysis of HuR expression on A2780 and OVCAR-3 nuclear (nu) and cytoplasmic (cyt) fractions. SNRP70 and GAPDH were utilized as markers of nuclear and cytoplasmic extracts respectively. The protein content were used as loading control.